NiO nanoparticle-based urea biosensor.
Identifieur interne : 000515 ( Main/Exploration ); précédent : 000514; suivant : 000516NiO nanoparticle-based urea biosensor.
Auteurs : RBID : pubmed:22947517English descriptors
- KwdEn :
- Biosensing Techniques (instrumentation), Conductometry (instrumentation), Enzymes, Immobilized (chemistry), Equipment Design, Equipment Failure Analysis, Metal Nanoparticles (chemistry), Metal Nanoparticles (ultrastructure), Nanotechnology (instrumentation), Nickel (chemistry), Reproducibility of Results, Sensitivity and Specificity, Urea (analysis), Urease (chemistry).
- MESH :
- chemical , analysis : Urea.
- chemical , chemistry : Enzymes, Immobilized, Nickel, Urease.
- chemistry : Metal Nanoparticles.
- instrumentation : Biosensing Techniques, Conductometry, Nanotechnology.
- ultrastructure : Metal Nanoparticles.
- Equipment Design, Equipment Failure Analysis, Reproducibility of Results, Sensitivity and Specificity.
Abstract
NiO nanoparticles (NiO-NPs) have been exploited successfully for the fabrication of a urea biosensor. A thin film of NiO nanoparticles deposited on an indium tin oxide (ITO) coated glass substrate serves as an efficient matrix for the immobilisation of urease (Ur), the specific enzyme for urea detection. The prepared bioelectrode (Ur/NiO-NP/ITO/glass) is utilised for urea sensing using cyclic voltammetry and UV-visible spectroscopy. NiO nanoparticles act as electro-catalytic species that are based on the shuttling of electrons between Ni(2+) and Ni(3+) in the octahedral site and result in an enhanced electrochemical current response. The prepared bioelectrode (Ur/NiO-NPs/ITO/glass) exhibits a high sensitivity of 21.3 μA/(mM (*) cm(2)) and a good linearity in a wide range (0.83-16.65 Mm) of urea concentrations with fast response time of 5s. The low value of the Michaelis-Menten constant (K(m)=0.34 mM) indicates the high affinity of Ur towards the analyte (urea). The high catalytic activity, along with the redox behaviour of NiO-NPs, makes it an efficient matrix for the realisation of a urea biosensor.
DOI: 10.1016/j.bios.2012.07.062
PubMed: 22947517
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">NiO nanoparticle-based urea biosensor.</title><author><name sortKey="Tyagi, Manisha" uniqKey="Tyagi M">Manisha Tyagi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Physics and Astrophysics, University of Delhi, Delhi 110007</wicri:regionArea></affiliation></author><author><name sortKey="Tomar, Monika" uniqKey="Tomar M">Monika Tomar</name></author><author><name sortKey="Gupta, Vinay" uniqKey="Gupta V">Vinay Gupta</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1016/j.bios.2012.07.062</idno><idno type="RBID">pubmed:22947517</idno><idno type="pmid">22947517</idno><idno type="wicri:Area/Main/Corpus">000A88</idno><idno type="wicri:Area/Main/Curation">000A88</idno><idno type="wicri:Area/Main/Exploration">000515</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term><term>Conductometry (instrumentation)</term><term>Enzymes, Immobilized (chemistry)</term><term>Equipment Design</term><term>Equipment Failure Analysis</term><term>Metal Nanoparticles (chemistry)</term><term>Metal Nanoparticles (ultrastructure)</term><term>Nanotechnology (instrumentation)</term><term>Nickel (chemistry)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Urea (analysis)</term><term>Urease (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Urea</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Enzymes, Immobilized</term><term>Nickel</term><term>Urease</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>Conductometry</term><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term><term>Equipment Failure Analysis</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">NiO nanoparticles (NiO-NPs) have been exploited successfully for the fabrication of a urea biosensor. A thin film of NiO nanoparticles deposited on an indium tin oxide (ITO) coated glass substrate serves as an efficient matrix for the immobilisation of urease (Ur), the specific enzyme for urea detection. The prepared bioelectrode (Ur/NiO-NP/ITO/glass) is utilised for urea sensing using cyclic voltammetry and UV-visible spectroscopy. NiO nanoparticles act as electro-catalytic species that are based on the shuttling of electrons between Ni(2+) and Ni(3+) in the octahedral site and result in an enhanced electrochemical current response. The prepared bioelectrode (Ur/NiO-NPs/ITO/glass) exhibits a high sensitivity of 21.3 μA/(mM (*) cm(2)) and a good linearity in a wide range (0.83-16.65 Mm) of urea concentrations with fast response time of 5s. The low value of the Michaelis-Menten constant (K(m)=0.34 mM) indicates the high affinity of Ur towards the analyte (urea). The high catalytic activity, along with the redox behaviour of NiO-NPs, makes it an efficient matrix for the realisation of a urea biosensor.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">22947517</PMID><DateCreated><Year>2012</Year><Month>12</Month><Day>18</Day></DateCreated><DateCompleted><Year>2013</Year><Month>05</Month><Day>30</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4235</ISSN><JournalIssue CitedMedium="Internet"><Volume>41</Volume><PubDate><Year>2013</Year><Month>Mar</Month><Day>15</Day></PubDate></JournalIssue><Title>Biosensors & bioelectronics</Title><ISOAbbreviation>Biosens Bioelectron</ISOAbbreviation></Journal><ArticleTitle>NiO nanoparticle-based urea biosensor.</ArticleTitle><Pagination><MedlinePgn>110-5</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bios.2012.07.062</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0956-5663(12)00504-0</ELocationID><Abstract><AbstractText>NiO nanoparticles (NiO-NPs) have been exploited successfully for the fabrication of a urea biosensor. A thin film of NiO nanoparticles deposited on an indium tin oxide (ITO) coated glass substrate serves as an efficient matrix for the immobilisation of urease (Ur), the specific enzyme for urea detection. The prepared bioelectrode (Ur/NiO-NP/ITO/glass) is utilised for urea sensing using cyclic voltammetry and UV-visible spectroscopy. NiO nanoparticles act as electro-catalytic species that are based on the shuttling of electrons between Ni(2+) and Ni(3+) in the octahedral site and result in an enhanced electrochemical current response. The prepared bioelectrode (Ur/NiO-NPs/ITO/glass) exhibits a high sensitivity of 21.3 μA/(mM (*) cm(2)) and a good linearity in a wide range (0.83-16.65 Mm) of urea concentrations with fast response time of 5s. The low value of the Michaelis-Menten constant (K(m)=0.34 mM) indicates the high affinity of Ur towards the analyte (urea). The high catalytic activity, along with the redox behaviour of NiO-NPs, makes it an efficient matrix for the realisation of a urea biosensor.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tyagi</LastName><ForeName>Manisha</ForeName><Initials>M</Initials><Affiliation>Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.</Affiliation></Author><Author ValidYN="Y"><LastName>Tomar</LastName><ForeName>Monika</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Vinay</ForeName><Initials>V</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>08</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biosens Bioelectron</MedlineTA><NlmUniqueID>9001289</NlmUniqueID><ISSNLinking>0956-5663</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Enzymes, Immobilized</NameOfSubstance></Chemical><Chemical><RegistryNumber>7OV03QG267</RegistryNumber><NameOfSubstance>Nickel</NameOfSubstance></Chemical><Chemical><RegistryNumber>8W8T17847W</RegistryNumber><NameOfSubstance>Urea</NameOfSubstance></Chemical><Chemical><RegistryNumber>C3574QBZ3Y</RegistryNumber><NameOfSubstance>nickel monoxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.5.1.5</RegistryNumber><NameOfSubstance>Urease</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Conductometry</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Enzymes, Immobilized</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Equipment Design</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Equipment Failure Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName><QualifierName MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanotechnology</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nickel</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Urea</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Urease</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>4</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>7</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>7</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2012</Year><Month>8</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0956-5663(12)00504-0</ArticleId><ArticleId IdType="doi">10.1016/j.bios.2012.07.062</ArticleId><ArticleId IdType="pubmed">22947517</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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